Western blot was performed using Anti-MEIS1 Monoclonal Antibody (Product # MA5-27191). A 50 kDa band and a 41kDa band corresponding to different isoforms of MEIS1 was observed in IMR-32, SK-N-AS, K-562, HeLa cell lines. The 50 kDa band corresponding to MEIS1 was also observed in Mouse Cerebellum, Mouse Lung, Rat Lung and Mouse Skeletal muscle. A non-characterized band corresponding to 58 kDa was observed in the cell lines tested. Modified Whole cell extracts (30 µg lysate) of IMR-32 (Lane 1), SK-A-NS (Lane 2), K-562 (Lane 3), Jurkat (Lane 4), HeLa (Lane 5), Daudi (Lane 6), tissue extracts of Mouse Cerebellum (Lane 7), Mouse Lung (Lane 8), Rat Lung (Lane 9), Mouse Skeletal Muscle (lane 10) and Mouse Spleen (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence Goat Anti-Mouse IgG Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).